The rally against Indian control on Monday is expected to be the biggest protest in two decades despite the curfew and heavy police presence.
Mirwaiz Umar Farooq, the chairman of Kashmir's main separatist alliance, and Syed Ali Shah Geelani, an Indian politician and member of the All Parties Hurriyat Conference, were detained in overnight raids in Srinagar, police said.
"Both have been detained for precautionary measures," a senior police official said.
One protester was shot dead by paramilitary soldiers and police when they opened fire late on Sunday as protesters started to gather.
Dozens of people have been killed in several weeks of protests sparked by a move by the government to give land to a Hindu shrine.
Strained relations
The rally will be in the Lal Chowk region of Srinagar, the summer capital, raising fears of further deaths.
A statement from the All Parties Hurriyat Conference said: "The people of Kashmir were ready to defy the curfew and carry out the march to protest against Indian occupation."
Police have killed at least 24 Muslim protesters and more than 500 have been injured in clashes in two weeks of demonstrations held in the Kashmir Valley.
The crisis has strained relations between India and Pakistan.
It has also raised fears of communal tension in the state, split between the Hindu-majority Jammu region and the Muslim Kashmir Valley.
A dispute over land for Hindu pilgrims visiting a shrine in Kashmir led to the rallies this month, boosting separatists who want India's only Muslim-majority region to secede.
With a general election approaching early next year, analysts say there is little chance of Manmohan Singh, the Indian prime minister, giving any concessions to the protesters.
In Kashmir, more than 43,000 people have been killed in unrest involving Indian troops and separatists since 1989. Human rights groups say at least 60,000 are either dead or missing.
Levels of violence in Kashmir have fallen in the past few years amid tighter Indian security and a tentative peace process between Pakistan and India.
Agencies
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